plasmid encoding t7 promoter Search Results


t7 promoter sequence  (Addgene inc)
94
Addgene inc t7 promoter sequence
T7 Promoter Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
t7 promoter sequence - by Bioz Stars, 2026-10
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dual t7 promoter system  (Addgene inc)
93
Addgene inc dual t7 promoter system
Dual T7 Promoter System, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+encoding+t7+promoter/Dual+opposing+T7+promoters+reporter+plasmid+(Plasmid+%23156457)/pm33408643-283-16-33
Average 93 stars, based on 1 article reviews
dual t7 promoter system - by Bioz Stars, 2026-10
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plasmid encoding t7 promoter  (Addgene inc)
92
Addgene inc plasmid encoding t7 promoter
( A ) V976L mutation emerged in 190366 virus stocks. ( B ) Pseudotyped particles were produced with full-length spike WT or the V967L mutant. Spike was detected in producer cells and pseudotyped by western blotting for FLAG. ( C ) Indicated cells were infected with viral pseudotyped in the presence or absence of trypsin. ( D ) Schematic overview of the dual-reporter fusion assay developed for this study. <t>T7</t> polymerase drives expression <t>of</t> <t>GFP</t> and luciferase separated by a P2A fusion peptide. ( E ) HEK 293T cells expressing receptor or, ( F) empty vector and T7-polymerase were combined with cells expressing spike and the T7-driven reporter. Luciferase was measured as a readout for cell fusion. Dotted lines indicate data from 1:4 ratio of receptor:spike cells. ( G ) Overview of 190366 spike with in silico predicted trypsin digest sites indicated. Location of V976L is indicated in green. ( H ) Concentrated viral pseudotyped were combined with a wide range or trypsin dilutions or ( I ) a fine range of trypsin dilutions and incubated at 37°C. Spike digestion was assessed by western blot for FLAG epitope.
Plasmid Encoding T7 Promoter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+encoding+t7+promoter/Reporter+Plasmid+Lacking+T7+Promoter+(Plasmid+%23156453)/bio_rxiv__2023__03__02__530738-123-0-34
Average 92 stars, based on 1 article reviews
plasmid encoding t7 promoter - by Bioz Stars, 2026-10
92/100 stars
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t7 promoter  (Addgene inc)
94
Addgene inc t7 promoter
( A ) V976L mutation emerged in 190366 virus stocks. ( B ) Pseudotyped particles were produced with full-length spike WT or the V967L mutant. Spike was detected in producer cells and pseudotyped by western blotting for FLAG. ( C ) Indicated cells were infected with viral pseudotyped in the presence or absence of trypsin. ( D ) Schematic overview of the dual-reporter fusion assay developed for this study. <t>T7</t> polymerase drives expression <t>of</t> <t>GFP</t> and luciferase separated by a P2A fusion peptide. ( E ) HEK 293T cells expressing receptor or, ( F) empty vector and T7-polymerase were combined with cells expressing spike and the T7-driven reporter. Luciferase was measured as a readout for cell fusion. Dotted lines indicate data from 1:4 ratio of receptor:spike cells. ( G ) Overview of 190366 spike with in silico predicted trypsin digest sites indicated. Location of V976L is indicated in green. ( H ) Concentrated viral pseudotyped were combined with a wide range or trypsin dilutions or ( I ) a fine range of trypsin dilutions and incubated at 37°C. Spike digestion was assessed by western blot for FLAG epitope.
T7 Promoter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+encoding+t7+promoter/T7+promoter+%2B+filler+DNA+reporter+plasmid+(Plasmid+%23156454)/pmc08476540-113-14-27
Average 94 stars, based on 1 article reviews
t7 promoter - by Bioz Stars, 2026-10
94/100 stars
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ppsg-iba series plasmids (which allows attachment of the 6xhistidine-tag to the fusion protein and expression from the bacteriophage t7 promoter)  (IBA Biotagnology)
90
IBA Biotagnology ppsg-iba series plasmids (which allows attachment of the 6xhistidine-tag to the fusion protein and expression from the bacteriophage t7 promoter)
( A ) V976L mutation emerged in 190366 virus stocks. ( B ) Pseudotyped particles were produced with full-length spike WT or the V967L mutant. Spike was detected in producer cells and pseudotyped by western blotting for FLAG. ( C ) Indicated cells were infected with viral pseudotyped in the presence or absence of trypsin. ( D ) Schematic overview of the dual-reporter fusion assay developed for this study. <t>T7</t> polymerase drives expression <t>of</t> <t>GFP</t> and luciferase separated by a P2A fusion peptide. ( E ) HEK 293T cells expressing receptor or, ( F) empty vector and T7-polymerase were combined with cells expressing spike and the T7-driven reporter. Luciferase was measured as a readout for cell fusion. Dotted lines indicate data from 1:4 ratio of receptor:spike cells. ( G ) Overview of 190366 spike with in silico predicted trypsin digest sites indicated. Location of V976L is indicated in green. ( H ) Concentrated viral pseudotyped were combined with a wide range or trypsin dilutions or ( I ) a fine range of trypsin dilutions and incubated at 37°C. Spike digestion was assessed by western blot for FLAG epitope.
Ppsg Iba Series Plasmids (Which Allows Attachment Of The 6xhistidine Tag To The Fusion Protein And Expression From The Bacteriophage T7 Promoter), supplied by IBA Biotagnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
ppsg-iba series plasmids (which allows attachment of the 6xhistidine-tag to the fusion protein and expression from the bacteriophage t7 promoter) - by Bioz Stars, 2026-10
90/100 stars
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expression plasmids using t7 promoter for heterologous expression in e. coli  (Merck KGaA)
90
Merck KGaA expression plasmids using t7 promoter for heterologous expression in e. coli
( A ) V976L mutation emerged in 190366 virus stocks. ( B ) Pseudotyped particles were produced with full-length spike WT or the V967L mutant. Spike was detected in producer cells and pseudotyped by western blotting for FLAG. ( C ) Indicated cells were infected with viral pseudotyped in the presence or absence of trypsin. ( D ) Schematic overview of the dual-reporter fusion assay developed for this study. <t>T7</t> polymerase drives expression <t>of</t> <t>GFP</t> and luciferase separated by a P2A fusion peptide. ( E ) HEK 293T cells expressing receptor or, ( F) empty vector and T7-polymerase were combined with cells expressing spike and the T7-driven reporter. Luciferase was measured as a readout for cell fusion. Dotted lines indicate data from 1:4 ratio of receptor:spike cells. ( G ) Overview of 190366 spike with in silico predicted trypsin digest sites indicated. Location of V976L is indicated in green. ( H ) Concentrated viral pseudotyped were combined with a wide range or trypsin dilutions or ( I ) a fine range of trypsin dilutions and incubated at 37°C. Spike digestion was assessed by western blot for FLAG epitope.
Expression Plasmids Using T7 Promoter For Heterologous Expression In E. Coli, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+encoding+t7+promoter/expression+plasmids+using+t7+promoter+for+heterologous+expression+in+e++coli/us10738316-140-9-14
Average 90 stars, based on 1 article reviews
expression plasmids using t7 promoter for heterologous expression in e. coli - by Bioz Stars, 2026-10
90/100 stars
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sequences corresponding to the t7 expression and ampr cassettes  (GenScript corporation)
90
GenScript corporation sequences corresponding to the t7 expression and ampr cassettes
( A ) V976L mutation emerged in 190366 virus stocks. ( B ) Pseudotyped particles were produced with full-length spike WT or the V967L mutant. Spike was detected in producer cells and pseudotyped by western blotting for FLAG. ( C ) Indicated cells were infected with viral pseudotyped in the presence or absence of trypsin. ( D ) Schematic overview of the dual-reporter fusion assay developed for this study. <t>T7</t> polymerase drives expression <t>of</t> <t>GFP</t> and luciferase separated by a P2A fusion peptide. ( E ) HEK 293T cells expressing receptor or, ( F) empty vector and T7-polymerase were combined with cells expressing spike and the T7-driven reporter. Luciferase was measured as a readout for cell fusion. Dotted lines indicate data from 1:4 ratio of receptor:spike cells. ( G ) Overview of 190366 spike with in silico predicted trypsin digest sites indicated. Location of V976L is indicated in green. ( H ) Concentrated viral pseudotyped were combined with a wide range or trypsin dilutions or ( I ) a fine range of trypsin dilutions and incubated at 37°C. Spike digestion was assessed by western blot for FLAG epitope.
Sequences Corresponding To The T7 Expression And Ampr Cassettes, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
sequences corresponding to the t7 expression and ampr cassettes - by Bioz Stars, 2026-10
90/100 stars
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plasmids containing the bc200 sequence fused to a t7 promoter  (GenScript corporation)
90
GenScript corporation plasmids containing the bc200 sequence fused to a t7 promoter
( A ) V976L mutation emerged in 190366 virus stocks. ( B ) Pseudotyped particles were produced with full-length spike WT or the V967L mutant. Spike was detected in producer cells and pseudotyped by western blotting for FLAG. ( C ) Indicated cells were infected with viral pseudotyped in the presence or absence of trypsin. ( D ) Schematic overview of the dual-reporter fusion assay developed for this study. <t>T7</t> polymerase drives expression <t>of</t> <t>GFP</t> and luciferase separated by a P2A fusion peptide. ( E ) HEK 293T cells expressing receptor or, ( F) empty vector and T7-polymerase were combined with cells expressing spike and the T7-driven reporter. Luciferase was measured as a readout for cell fusion. Dotted lines indicate data from 1:4 ratio of receptor:spike cells. ( G ) Overview of 190366 spike with in silico predicted trypsin digest sites indicated. Location of V976L is indicated in green. ( H ) Concentrated viral pseudotyped were combined with a wide range or trypsin dilutions or ( I ) a fine range of trypsin dilutions and incubated at 37°C. Spike digestion was assessed by western blot for FLAG epitope.
Plasmids Containing The Bc200 Sequence Fused To A T7 Promoter, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
plasmids containing the bc200 sequence fused to a t7 promoter - by Bioz Stars, 2026-10
90/100 stars
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singlestranded dna "blue" t7 promoter plasmids  (Kemper GmbH)
90
Kemper GmbH singlestranded dna "blue" t7 promoter plasmids
( A ) V976L mutation emerged in 190366 virus stocks. ( B ) Pseudotyped particles were produced with full-length spike WT or the V967L mutant. Spike was detected in producer cells and pseudotyped by western blotting for FLAG. ( C ) Indicated cells were infected with viral pseudotyped in the presence or absence of trypsin. ( D ) Schematic overview of the dual-reporter fusion assay developed for this study. <t>T7</t> polymerase drives expression <t>of</t> <t>GFP</t> and luciferase separated by a P2A fusion peptide. ( E ) HEK 293T cells expressing receptor or, ( F) empty vector and T7-polymerase were combined with cells expressing spike and the T7-driven reporter. Luciferase was measured as a readout for cell fusion. Dotted lines indicate data from 1:4 ratio of receptor:spike cells. ( G ) Overview of 190366 spike with in silico predicted trypsin digest sites indicated. Location of V976L is indicated in green. ( H ) Concentrated viral pseudotyped were combined with a wide range or trypsin dilutions or ( I ) a fine range of trypsin dilutions and incubated at 37°C. Spike digestion was assessed by western blot for FLAG epitope.
Singlestranded Dna "Blue" T7 Promoter Plasmids, supplied by Kemper GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+encoding+t7+promoter/single+stranded+dna++blue%E2%80%99+t7+promoter+plasmids/pm02563567-162-4-4
Average 90 stars, based on 1 article reviews
singlestranded dna "blue" t7 promoter plasmids - by Bioz Stars, 2026-10
90/100 stars
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dsrna using the t7 ribomax kit  (Promega)
90
Promega dsrna using the t7 ribomax kit
( A ) V976L mutation emerged in 190366 virus stocks. ( B ) Pseudotyped particles were produced with full-length spike WT or the V967L mutant. Spike was detected in producer cells and pseudotyped by western blotting for FLAG. ( C ) Indicated cells were infected with viral pseudotyped in the presence or absence of trypsin. ( D ) Schematic overview of the dual-reporter fusion assay developed for this study. <t>T7</t> polymerase drives expression <t>of</t> <t>GFP</t> and luciferase separated by a P2A fusion peptide. ( E ) HEK 293T cells expressing receptor or, ( F) empty vector and T7-polymerase were combined with cells expressing spike and the T7-driven reporter. Luciferase was measured as a readout for cell fusion. Dotted lines indicate data from 1:4 ratio of receptor:spike cells. ( G ) Overview of 190366 spike with in silico predicted trypsin digest sites indicated. Location of V976L is indicated in green. ( H ) Concentrated viral pseudotyped were combined with a wide range or trypsin dilutions or ( I ) a fine range of trypsin dilutions and incubated at 37°C. Spike digestion was assessed by western blot for FLAG epitope.
Dsrna Using The T7 Ribomax Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
dsrna using the t7 ribomax kit - by Bioz Stars, 2026-10
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protein gene-carrying plasmid (t7 promotor)  (Promega)
90
Promega protein gene-carrying plasmid (t7 promotor)
( A ) V976L mutation emerged in 190366 virus stocks. ( B ) Pseudotyped particles were produced with full-length spike WT or the V967L mutant. Spike was detected in producer cells and pseudotyped by western blotting for FLAG. ( C ) Indicated cells were infected with viral pseudotyped in the presence or absence of trypsin. ( D ) Schematic overview of the dual-reporter fusion assay developed for this study. <t>T7</t> polymerase drives expression <t>of</t> <t>GFP</t> and luciferase separated by a P2A fusion peptide. ( E ) HEK 293T cells expressing receptor or, ( F) empty vector and T7-polymerase were combined with cells expressing spike and the T7-driven reporter. Luciferase was measured as a readout for cell fusion. Dotted lines indicate data from 1:4 ratio of receptor:spike cells. ( G ) Overview of 190366 spike with in silico predicted trypsin digest sites indicated. Location of V976L is indicated in green. ( H ) Concentrated viral pseudotyped were combined with a wide range or trypsin dilutions or ( I ) a fine range of trypsin dilutions and incubated at 37°C. Spike digestion was assessed by western blot for FLAG epitope.
Protein Gene Carrying Plasmid (T7 Promotor), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+encoding+t7+promoter/protein+gene+carrying+plasmid++t7+promoter+/pm29664612-212-10-21
Average 90 stars, based on 1 article reviews
protein gene-carrying plasmid (t7 promotor) - by Bioz Stars, 2026-10
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pgem plasmid having t7 promoter used for in-vitro transcription assay  (Promega)
90
Promega pgem plasmid having t7 promoter used for in-vitro transcription assay
( A ) V976L mutation emerged in 190366 virus stocks. ( B ) Pseudotyped particles were produced with full-length spike WT or the V967L mutant. Spike was detected in producer cells and pseudotyped by western blotting for FLAG. ( C ) Indicated cells were infected with viral pseudotyped in the presence or absence of trypsin. ( D ) Schematic overview of the dual-reporter fusion assay developed for this study. <t>T7</t> polymerase drives expression <t>of</t> <t>GFP</t> and luciferase separated by a P2A fusion peptide. ( E ) HEK 293T cells expressing receptor or, ( F) empty vector and T7-polymerase were combined with cells expressing spike and the T7-driven reporter. Luciferase was measured as a readout for cell fusion. Dotted lines indicate data from 1:4 ratio of receptor:spike cells. ( G ) Overview of 190366 spike with in silico predicted trypsin digest sites indicated. Location of V976L is indicated in green. ( H ) Concentrated viral pseudotyped were combined with a wide range or trypsin dilutions or ( I ) a fine range of trypsin dilutions and incubated at 37°C. Spike digestion was assessed by western blot for FLAG epitope.
Pgem Plasmid Having T7 Promoter Used For In Vitro Transcription Assay, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
pgem plasmid having t7 promoter used for in-vitro transcription assay - by Bioz Stars, 2026-10
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Image Search Results


( A ) V976L mutation emerged in 190366 virus stocks. ( B ) Pseudotyped particles were produced with full-length spike WT or the V967L mutant. Spike was detected in producer cells and pseudotyped by western blotting for FLAG. ( C ) Indicated cells were infected with viral pseudotyped in the presence or absence of trypsin. ( D ) Schematic overview of the dual-reporter fusion assay developed for this study. T7 polymerase drives expression of GFP and luciferase separated by a P2A fusion peptide. ( E ) HEK 293T cells expressing receptor or, ( F) empty vector and T7-polymerase were combined with cells expressing spike and the T7-driven reporter. Luciferase was measured as a readout for cell fusion. Dotted lines indicate data from 1:4 ratio of receptor:spike cells. ( G ) Overview of 190366 spike with in silico predicted trypsin digest sites indicated. Location of V976L is indicated in green. ( H ) Concentrated viral pseudotyped were combined with a wide range or trypsin dilutions or ( I ) a fine range of trypsin dilutions and incubated at 37°C. Spike digestion was assessed by western blot for FLAG epitope.

Journal: bioRxiv

Article Title: Isolation of ACE2-dependent and -independent sarbecoviruses from Chinese horseshoe bats

doi: 10.1101/2023.03.02.530738

Figure Lengend Snippet: ( A ) V976L mutation emerged in 190366 virus stocks. ( B ) Pseudotyped particles were produced with full-length spike WT or the V967L mutant. Spike was detected in producer cells and pseudotyped by western blotting for FLAG. ( C ) Indicated cells were infected with viral pseudotyped in the presence or absence of trypsin. ( D ) Schematic overview of the dual-reporter fusion assay developed for this study. T7 polymerase drives expression of GFP and luciferase separated by a P2A fusion peptide. ( E ) HEK 293T cells expressing receptor or, ( F) empty vector and T7-polymerase were combined with cells expressing spike and the T7-driven reporter. Luciferase was measured as a readout for cell fusion. Dotted lines indicate data from 1:4 ratio of receptor:spike cells. ( G ) Overview of 190366 spike with in silico predicted trypsin digest sites indicated. Location of V976L is indicated in green. ( H ) Concentrated viral pseudotyped were combined with a wide range or trypsin dilutions or ( I ) a fine range of trypsin dilutions and incubated at 37°C. Spike digestion was assessed by western blot for FLAG epitope.

Article Snippet: Plasmid encoding T7-promoter driven dual reporter GFP and luciferase was generated by cloning firefly luciferase downstream of GFP in pUC19-T7-IRES-GFP. pUC19 - T7 pro - IRES - EGFP was a gift from Fei Chen (Addgene plasmid # 138586; http://n2t.net/addgene:138586 ; RRID: Addgene_138586).

Techniques: Mutagenesis, Produced, Western Blot, Infection, Single Vesicle Fusion Assay, Expressing, Luciferase, Plasmid Preparation, In Silico, Incubation, FLAG-tag